Investigation of OprD Porin Protein Levels in Carbapenem-Resistant Pseudomonas aeruginosa Isolates
نویسندگان
چکیده
منابع مشابه
Investigation of OprD Porin Protein Levels in Carbapenem-Resistant Pseudomonas aeruginosa Isolates.
BACKGROUND The Pseudomonas aeruginosa porin OprD is a substrate-specific porin that facilitates the diffusion of basic amino acids, small peptides, and carbapenems into the cell. OprD-mediated resistance occurs as a result of decreased transcriptional expression of oprD and/or loss of function mutations that disrupt protein activity. OBJECTIVES In this study, we examined the level of oprD exp...
متن کاملinvestigation of oprd porin protein levels in carbapenem-resistant pseudomonas aeruginosa isolates
conclusions although the data support the idea that the basic mechanism of imipenem resistance could be via the loss of oprd, they do not fully explain the role of oprd and indicate that other mechanisms may play an important role. additionally, the significant decrease in the oprd levels in mdr strains suggests that oprd also plays a role in the emergence of both carbapenem and non-carbapenem ...
متن کاملInterplay of efflux system, ampC, and oprD expression in carbapenem resistance of Pseudomonas aeruginosa clinical isolates.
Carbapenems are important agents for the therapy of infections due to multidrug-resistant Pseudomonas aeruginosa; the development of carbapenem resistance hampers effective therapeutic options. To assess the mechanisms leading to resistance, 33 clinical isolates with differing degrees of carbapenem susceptibility were analyzed for the expression of the chromosomal beta-lactamase (ampC), the por...
متن کاملDetection of outer membrane porin protein, an imipenem influx channel, in Pseudomonas aeruginosa clinical isolates.
Decreased permeability to imipenem is the most frequent mechanism of imipenem resistance in Pseudomonas aeruginosa. We have determined the presence of OprD porin protein, an imipenem influx channel, in 70 carbapenem-resistant P. aeruginosa clinical isolates by Western blot analysis using rabbit anti-OprD polyclonal antibody. Ninety-eight percent (54 of 55 isolates) of imipenem-and meropenem-res...
متن کاملCarbapenem and Fluoroquinolone Resistance in Multidrug Resistant Pseudomonas aeruginosa Isolates from Al-Zahra Hospital, Isfahan, Iran
Introduction: The major resistance mechanisms of Pseudomonas aeruginosa to fluoroquinolones and carbapenems are associated with the mutations in the genes gyrA and oprD encoding type II topoisomerases (DNA gyrase) and OprD porin, respectively. Method: In this cross-sectional study, sixty five clinical samples were collected from patients hospitalized in Al-Zahra Hospital of Isfahan, Iran. Susce...
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ژورنال
عنوان ژورنال: Jundishapur Journal of Microbiology
سال: 2015
ISSN: 2008-3645,2008-4161
DOI: 10.5812/jjm.25952